Estimation of Metronidazole, Furazolidone and Loperamide in Veterinary Formulation by Rp-hplc

A RP-HPLC method for the simultaneous estimation of Metronidazole (MTZ), Furazolidone (FZD) and Loperamide (LOP) was developed in the present study. The chromatographic elution was carried out on Phenomenex C18 column (150×4.6mm) with 5μm particle size using Acetonitrile: Double distilled water in the ratio of 10:90% v/v as mobile phase and the detection was carried out at 230nm. The Retention times were found to be 5.35, 6.84 and 16.59 for MTZ, LOP and FZD respectively. The developed method was validated according to ICH guidelines. The method was found to be linear over a concentration range of 160-240 μg/ml for MTZ and LOP and 60-140 μg/ml for FZD respectively. Accuracy of the method was assessed by recovery studies and the percentage recovery was found to be within 99.34 – 100.90% w/w. The proposed method was simple, accurate, precise and sensitive. Hence the method can be released for routine quality control analysis of these drugs in Pharmaceutical and Veterinary formulations.